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Biology 30 · Toolkits

Reading pedigrees

A pedigree question is a logic puzzle with four possible answers: autosomal or sex-linked, dominant or recessive. This page gives the symbols, the tests that eliminate each option, a worked example, and how to get carrier probabilities out of a chart.

The symbols

The idea: squares are male, circles are female, shaded means affected. Generations are numbered with Roman numerals from the top, individuals left to right.

SymbolMeans
SquareMale
CircleFemale
ShadedShows the trait
UnshadedDoes not show the trait — may still be a carrier
Horizontal line between two symbolsA mating
Vertical line down to a sibling barTheir children, oldest on the left

The symbols are on the Biology 30 data pages, so you do not have to memorize them — but the reasoning below is not, and that is where the marks are.

Four tests, in order

The idea: settle dominant or recessive first, then decide whether it sits on an autosome or the X.

  1. Two unaffected parents with an affected child → recessive. The child has two copies of an allele neither parent shows, so both parents are carriers. A trait that skips generations is recessive.
  2. Two affected parents with an unaffected child → dominant. Both parents must be heterozygous, and the unaffected child got the recessive allele twice.
  3. Then test sex linkage, for a recessive trait: an affected female must have an affected father, because she needs a recessive allele on each X and one X comes from him. An affected daughter with an unaffected father rules out X-linked recessive — it is autosomal.
  4. And for a dominant trait: an affected father passes his X to every daughter, so with X-linked dominant all his daughters are affected and his sons are unaffected unless the mother passes it. An affected father with an unaffected daughter rules out X-linked dominant.

Supporting clues, not proof

  • Far more affected males than females suggests X-linked recessive, which is why red-green colour blindness and hemophilia show that pattern.
  • The trait appears in every generation suggests dominant; skipping generations suggests recessive.
  • Numbers in a small family prove nothing on their own — always back a claim with one of the four tests.

A worked pedigree

The idea: name the mode of inheritance, then assign genotypes from the affected individuals outwards.

I II 1 2 1 2 3 unaffected affected
Generation I: two unaffected parents. Generation II: an unaffected son, an affected daughter, an unaffected daughter.
  1. Recessive or dominant? Two unaffected parents (I-1, I-2) have an affected child (II-2), so the trait is recessive and both parents are carriers.
  2. Autosomal or X-linked? II-2 is an affected female. If the trait were X-linked recessive she would need Xa from her father, so he would be affected — and he is not. So it is autosomal recessive.
  3. Genotypes: II-2 is aa. I-1 and I-2 are both Aa. II-1 and II-3 are unaffected, so each is AA or Aa.
  4. State the reasoning, not just the conclusion — a written-response question asks you to justify, and the justification is the mark.

Carrier probabilities

The idea: knowing someone is unaffected removes one box from the Punnett square, which changes the odds.

Worked: what is the chance II-3 is a carrier?

Her parents are Aa × Aa, so the boxes are AA, Aa, Aa, aa. She is unaffected, so aa is out, leaving three equally likely boxes, two of which are Aa.

The probability is 2/3. The instinctive answer of 1/2 forgets that being unaffected is information.

Worked: what is the chance the next child is an affected boy?

Affected is 1/4, and a boy is 1/2, and the events are independent: 1/4 × 1/2 = 1/8.

Traps

The idea: most wrong pedigree answers come from four habits.

  • Guessing from the number of affected males. It is a hint; the four tests are proof.
  • Writing X-linked genotypes without the chromosome. Use XAXa and XaY, never Aa and aY.
  • Assuming an unaffected person is homozygous dominant. For a recessive trait they may be a carrier, and often the question is about exactly that.
  • Forgetting that marrying into the family brings new alleles. An unrelated partner is usually assumed homozygous dominant unless the chart shows otherwise — say that you are assuming it.

Punnett square toolkit Practise genetics